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    HLA-F

    <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
    • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
    • <p>Figure 2:Western blot analysis using HLA-F mAb against human HLA-F (AA: 22-305) recombinant protein. (Expected MW is 35.6 kDa)</p>
    • <p>Figure 3:Western blot analysis using HLA-F mAb against HEK293-6e (1) and HLA-F (AA: 22-305)-hIgGFc transfected HEK293-6e (2) cell lysate.</p>
    • <p>Figure 4:Western blot analysis using HLA-F mouse mAb against Mouse  Liver (1) tissue lysate.</p>
    • <p>Figure 5:Flow cytometric analysis of Jurkat cells using HLA-F mouse mAb (green) and negative control (red).</p>

    圖片

    • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

    • Figure 2:Western blot analysis using HLA-F mAb against human HLA-F (AA: 22-305) recombinant protein. (Expected MW is 35.6 kDa)

    • Figure 3:Western blot analysis using HLA-F mAb against HEK293-6e (1) and HLA-F (AA: 22-305)-hIgGFc transfected HEK293-6e (2) cell lysate.

    • Figure 4:Western blot analysis using HLA-F mouse mAb against Mouse Liver (1) tissue lysate.

    • Figure 5:Flow cytometric analysis of Jurkat cells using HLA-F mouse mAb (green) and negative control (red).

    HLA-F
    Catalog#   :  32261
    Size/Concentration   :  1.0mg/ml
    Price:
    ¥ 2557.5
    瀏覽量:
    1
    Instruction:
    Specification
    Entrez GeneID3134
    AliasesHLAF; CDA12; HLA-5.4; HLA-CDA12
    Clone#3F11F4
    MW39kDa
    Host/IsotypeMouse IgG2b
    Storage4℃; -20℃ for long term storage
    Species ReactivityHuman; Mouse;
    ImmunogenPurified recombinant fragment of human HLA-F (AA: 22-305) expressed in E. Coli.
    FormulationPurified antibody in PBS with 0.05% sodium azide
    Application
    ELISA1/10000
    WB1/500 - 1/2000
    FCM1/200 - 1/400
    Description
    This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.
    References
    1.Hum Reprod. 2020 Mar 27;35(3):705-717.
    2.Int J Mol Sci. 2019 Nov 8;20(22):5572.
    Sequence

    extra(22-305)


    Diagram
    Elisa

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

    Western Blot

    Figure 2:Western blot analysis using HLA-F mAb against human HLA-F (AA: 22-305) recombinant protein. (Expected MW is 35.6 kDa)

    Western Blot

    Figure 3:Western blot analysis using HLA-F mAb against HEK293-6e (1) and HLA-F (AA: 22-305)-hIgGFc transfected HEK293-6e (2) cell lysate.

     

    Western Blot

    Figure 4:Western blot analysis using HLA-F mouse mAb against Mouse Liver (1) tissue lysate.

    Flow cytometric

    Figure 5:Flow cytometric analysis of Jurkat cells using HLA-F mouse mAb (green) and negative control (red).

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