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    BRAF

    <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
    • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
    • <p>Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)</p>
    • <p>Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.</p><br />
    • <p>Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).</p>
    • <p>Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.</p>
    • <p>Figure 6:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.</p>

    圖片

    • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

    • Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)

    • Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.


    • Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).

    • Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.

    • Figure 6:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.

    BRAF
    Catalog#   :  32150
    Size/Concentration   :  1.0mg/ml
    Price:
    ¥ 2557.5
    瀏覽量:
    1
    Instruction:
    Specification
    Entrez GeneID673
    AliasesNS7; B-raf; BRAF1; RAFB1; B-RAF1
    Clone#2A10D7
    MW84.4kda
    Host/IsotypeMouse IgG1
    Storage4℃; -20℃ for long term storage
    Species ReactivityHuman; Rat;
    ImmunogenPurified recombinant fragment of human BRAF (AA: 299-447) expressed in HEK293-6e.
    FormulationPurified antibody in PBS with 0.05% sodium azide
    Application
    ELISA1/10000
    WB1/500 - 1/2000
    FCM1/200 - 1/400
    IHC1/200 - 1/1000
    Description
    This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
    References
    1,Proc Natl Acad Sci U S A. 2020 Dec 8;117(49):31105-31113.
    2,Sci Rep. 2020 Oct 9;10(1):16943.
    Sequence

    299-447


    Diagram
    Elisa

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

    Western Blot

    Figure 2:Western blot analysis using BRAF mAb against human BRAF (AA: 299-447) recombinant protein. (Expected MW is 46kDa)

    Western Blot

    Figure 3:Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.

     

    Flow cytometric

    Figure 4:Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).

    Immunohistochemical analysis

    Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BRAF mouse mAb with DAB staining.

    Immunohistochemical analysis

    Figure 6:Immunohistochemical analysis of paraffin-embedded cerebellar tissues using BRAF mouse mAb with DAB staining.

     

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