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    GZMK

    <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
    • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
    • <p>Figure 2:Western blot analysis using GZMK mAb against human GZMK (AA: 27-264) recombinant protein. (Expected MW is 28.7kDa)</p>
    • <p>Figure 3:Western blot analysis using GZMK mAb against HEK293-6e (1) and GZMK (AA: 27-264)-hIgGFc transfected HEK293-6e (2) cell lysate.</p>
    • <p>Figure 4:Flow cytometric analysis of Jurkat cells using GZMK mouse mAb (green) and negative control (red).</p>
    • <p>Figure 5:Flow cytometric analysis of MOLT4 cells using GZMK mouse mAb (green) and negative control (red).</p>
    • <p>Figure 6:Immunohistochemical analysis of paraffin-embedded tonsil tissues using GZMK mouse mAb with DAB staining.</p>

    圖片

    • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

    • Figure 2:Western blot analysis using GZMK mAb against human GZMK (AA: 27-264) recombinant protein. (Expected MW is 28.7kDa)

    • Figure 3:Western blot analysis using GZMK mAb against HEK293-6e (1) and GZMK (AA: 27-264)-hIgGFc transfected HEK293-6e (2) cell lysate.

    • Figure 4:Flow cytometric analysis of Jurkat cells using GZMK mouse mAb (green) and negative control (red).

    • Figure 5:Flow cytometric analysis of MOLT4 cells using GZMK mouse mAb (green) and negative control (red).

    • Figure 6:Immunohistochemical analysis of paraffin-embedded tonsil tissues using GZMK mouse mAb with DAB staining.

    GZMK
    Catalog#   :  32147
    Size/Concentration   :  1.0mg/ml
    Price:
    ¥ 2557.5
    瀏覽量:
    1
    Instruction:
    Specification
    Entrez GeneID3003
    AliasesTRYP2
    Clone#5B4A5
    MW29kDa
    Host/IsotypeMouse IgG2b
    Storage4℃; -20℃ for long term storage
    Species ReactivityHuman;
    ImmunogenPurified recombinant fragment of human GZMK (AA: 27-264) expressed in E. Coli.
    FormulationPurified antibody in PBS with 0.05% sodium azide
    Application
    ELISA1/10000
    WB1/500 - 1/2000
    FCM1/200 - 1/400
    IHC1/200 - 1/1000
    Description
    This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes.
    References
    1,Brain. 2019 Nov 1;142(11):3411-3427.
    2,J Invest Dermatol. 2019 Apr;139(4):930-939.
    Sequence

    27-264


    Diagram
    Elisa

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

    Western Blot

    Figure 2:Western blot analysis using GZMK mAb against human GZMK (AA: 27-264) recombinant protein. (Expected MW is 28.7kDa)

    Western Blot

    Figure 3:Western blot analysis using GZMK mAb against HEK293-6e (1) and GZMK (AA: 27-264)-hIgGFc transfected HEK293-6e (2) cell lysate.

     

    Flow cytometric

    Figure 4:Flow cytometric analysis of Jurkat cells using GZMK mouse mAb (green) and negative control (red).

    Flow cytometric

    Figure 5:Flow cytometric analysis of MOLT4 cells using GZMK mouse mAb (green) and negative control (red).

    Immunohistochemical analysis

    Figure 6:Immunohistochemical analysis of paraffin-embedded tonsil tissues using GZMK mouse mAb with DAB staining.

     

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