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    CD109

    <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
    • <p>Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)</p>
    • <p>Figure 2:Western blot analysis using CD109 mAb against human CD109 (AA: extra 1274-1421) recombinant protein. (Expected MW is 42.3 kDa)</p>
    • <p>Figure 3:Western blot analysis using CD109 mAb against HEK293-6e (1) and CD109 (AA: extra 1274-1421)-hIgGFc transfected HEK293-6e (2) cell lysate.</p>
    • <p>Figure 4:Immunofluorescence analysis of Hela cells using CD109 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)</p>
    • <p>Figure 5:Flow cytometric analysis of Jurkat cells using CD109 mouse mAb (green) and negative control (red).</p>

    圖片

    • Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

    • Figure 2:Western blot analysis using CD109 mAb against human CD109 (AA: extra 1274-1421) recombinant protein. (Expected MW is 42.3 kDa)

    • Figure 3:Western blot analysis using CD109 mAb against HEK293-6e (1) and CD109 (AA: extra 1274-1421)-hIgGFc transfected HEK293-6e (2) cell lysate.

    • Figure 4:Immunofluorescence analysis of Hela cells using CD109 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

    • Figure 5:Flow cytometric analysis of Jurkat cells using CD109 mouse mAb (green) and negative control (red).

    CD109
    Catalog#   :  32142
    Size/Concentration   :  1.0mg/ml
    Price:
    ¥ 2557.5
    瀏覽量:
    1
    Instruction:
    Specification
    Entrez GeneID135228
    Aliasesp180; r150; CPAMD7
    Clone#1A3D3
    MW161.7kDa
    Host/IsotypeMouse IgG1
    Storage4℃; -20℃ for long term storage
    Species ReactivityHuman;
    ImmunogenPurified recombinant fragment of human CD109 (AA: extra 1274-1421) expressed in E. Coli.
    FormulationPurified antibody in PBS with 0.05% sodium azide
    Application
    ELISA1/10000
    WB1/500 - 1/2000
    ICC1/20 - 1/100
    FCM1/200 - 1/400
    Description
    This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene.
    References
    1.Cancer Sci. 2020 May;111(5):1652-1662.
    2.Diagn Pathol. 2015 Aug 7;10:137.
    Sequence

    extra(1274-1421)


    Diagram
    Elisa

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

    Western Blot

    Figure 2:Western blot analysis using CD109 mAb against human CD109 (AA: extra 1274-1421) recombinant protein. (Expected MW is 42.3 kDa)

    Western Blot

    Figure 3:Western blot analysis using CD109 mAb against HEK293-6e (1) and CD109 (AA: extra 1274-1421)-hIgGFc transfected HEK293-6e (2) cell lysate.

     

    Immunofluorescence analysis

    Figure 4:Immunofluorescence analysis of Hela cells using CD109 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

    Flow cytometric

    Figure 5:Flow cytometric analysis of Jurkat cells using CD109 mouse mAb (green) and negative control (red).

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